20  Agarose gels for PCR product verfication

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20.1

20.2 Do probiotic capsules contain Lactobacillus acidophilus?

Lactobacillus acidophilus probiotic capsules are widely available in supermarkets and health food shops as health-promoting supplements. To verify the identity of the bacteria within these capsules, primers specific to Lactobacillus acidophilus were designed and used in a PCR reaction. Three cell extracts were prepared from three bacterial colonies: two isolated from probiotic capsules, and one from E. coli, which was included as a negative control as it is not expected to be amplified by the primers. Four PCR reaction tubes were prepared, one for each cell extract and a forth as a no-template negative control. These were loaded on to an electrophoresis gel along with a 100 base pair DNA marker ladder

Primers specific to Lactobacillus acidophilus were designed and and used in a PCR reaction to confirm the identity of the bacteria isolated from probiotic capsules. E.coli was used as a negative control. The lanes from left to right are: 100 bp ladder, Lacidophilus colony 1, Lacidophilus colony 2, E.coli colony, no template control.

Primers specific to Lactobacillus acidophilus were designed and and used in a PCR reaction to confirm the identity of the bacteria isolated from probiotic capsules. E.coli was used as a negative control. The lanes from left to right are: 100 bp ladder, Lacidophilus colony 1, Lacidophilus colony 2, E.coli colony, no template control.

20.3 Is this bioremediation product Pseudomonas putida?

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Primers specific to Pseudomonas putida were designed and and used in a PCR reaction to confirm the identity of the bacteria isolated from a bioremediation product. E.coli was used as a negative control. The lanes from left to right are: 100 bp ladder, Pseudomonas putida colony 1, Pseudomonas putida colony 2, E.coli colony, no template control.

Primers specific to Pseudomonas putida were designed and and used in a PCR reaction to confirm the identity of the bacteria isolated from a bioremediation product. E.coli was used as a negative control. The lanes from left to right are: 100 bp ladder, Pseudomonas putida colony 1, Pseudomonas putida colony 2, E.coli colony, no template control.

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