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26C
BIO1 Food and Fuel
Pseudomonas putida is a hydrocarbon-degrading bacterium which can be used for cleaning up oil-contaminated soil.
Prac 1 check the identity of Pseudomonas putida
- Gram staining of your cultures
- an oxidase test.
- growth on a hydrocarbon substrate
- PCR analysed by gel electrophoresis. The expected size of the PCR product is 618 bp. The expectation is that you will see a band of this size for your Pseudomonas putida samples and not for E. coli or the water control
Prac 2 Assay the number of bacteria with spot plates
Response: Number of bacteria Two Explanatory: soil: contaminated with hexadecane or not temperatuire: 4 degrees or 25 degrees