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26C
BIO1 Food and Fuel
🟩 Pseudomonas putida is a hydrocarbon-degrading bacterium which can be used for cleaning up oil-contaminated soil.
Prac 1 check the identity of Pseudomonas putida
- Gram staining of your cultures
- an oxidase test.
- growth on a hydrocarbon substrate
- PCR analysed by gel electrophoresis. The expected size of the PCR product is 618 bp. The expectation is that you will see a band of this size for your Pseudomonas putida samples and not for E. coli or the water control
Prac 2 Assay the number of bacteria with spot plates
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Response: Number of bacteria Two Explanatory: soil: contaminated with hexadecane or not temperatuire: 4 degrees or 25 degrees
Prac 3 Design an experiment that uses methods from prac 2
BIO2 Health and disease
Prac 1
🟩 #### Prac 2
Prac 3
BCH
Prac 1
Prac 2
Prac 3
BMS Anitbiotics and antimicrobial resistance
Prac 1
Prac 2
Prac 3
66I
Biomedical sciences
flow cytometry to assess the effect of LPS and E. coli on TNFalpha production in macrophages
🟩 SDS-PAGE and western blot to assess TNFalpha production in the whole cell population across the media, LPS, and E. coli stimulated macrophage samples.
assess size of the protein band in comparison to TNFalpha, and the strength of the protein signal in each sample